Analysis of Potential Bioactive Peptides from Giant Grouper (Epinephelus lanceolatus) Roe Protein
| Autor: | Fenny Crista Anastasia Panjaitan, 柯芬妮 |
|---|---|
| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 Major proteins contained in dried giant grouper roe such as vitellogenin (from Epinephelus coioides; NCBI accession number: AAW29031.1), apolipoprotein A-1 precursor (from Epinephelus coioides; NCBI accession number: ACI01807.1) and apolipoprotein E (from Epinephelus bruneus; NCBI accession number: AEB31283.1) were characterized through compiled proteomics techniques (SDS-PAGE, in-gel digestion, mass spectrometry and on-line Mascot database analysis). These proteins were subjected to in silico analysis using BLAST (Basic Local Alignment Search Tool) and BIOPEP-UWM database. Sequence similarity search by BLAST revealed that the aligned vitellogenin sequences from Epinephelus coioides and Epinephelus lanceolatus share 70% identity. Moreover, prediction of potential bioactivities through BIOPEP-UWM database resulted in numerous bioactive peptides. Pepsin (pH >2) was predicted to be the most promising enzyme for the production of bioactive peptides. In vitro analysis confirmed that pepsin hydrolysates of giant grouper roe exhibited the highest ACE-I inhibition (34.09 ± 1.83%) compared to roe hydrolysates obtained from bromelain (31.15 ± 4.65%) and papain (21.51 ± 1.41%) proteolysis at 2 mg/ml concentration. However, roe hydrolyzed by papain and bromelain showed better DPPH scavenging activity (21.36 ± 0.37%) and reducing power activity (0.151 ± 0.040%), respectively, than did pepsin hydrolysates. Overall, the prediction through in silico approach was corresponded to in vitro analysis in this study. It thus confirmed the potentiality of giant grouper roe as raw material for the generation of nutraceutical or pharmaceutical ingredients. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|