Research of the artificial seeding technique of Sargassum ilicifolium
| Autor: | Yeh, Han-Yang, 葉翰揚 |
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| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 This study focused on the S. ilicifolium (Pheaophyta), which was the dominant species in northeast of Taiwan, provided the food and habitat for aquatic organisms. Recently, researchers in recent years had also discovered that after processing and extraction, various bioactive ingredients could be obtained from S. ilicifolium, therefore, increases its economic value. This study first investigated the habitat, growth performance of S. ilicifolium and water quality in the yield. Next, explored the best condition of environmental factor, includes temperature and light intensity on eggs and sperms release, fertilization rate, survival rate as well as zygotes development. This technique could provide the macroalgae product for the industry, even decreased the environmental impact of over exploitation. In the beginning, observed daily from the seashore of Zhong-Zheng Dist., Keelung City in March 2016. Following the variation of photoperiod increasingly (light more), S. ilicifolium growth continuously. Until July, algae getting mature and had the probability to release eggs and sperms. Furthermore, in order to confirm the identity of S. ilicifolium. This study used not only morphology also genetic screening included Riulose-1, 5-bisphosphate carboxylase/oxygenase spacer, RuBisCO and Internal transcribed spacer II, ITS-2 to distinguish. The result showed that 25℃ obtained the maximum egg release with a value 73.03 ± 10.94 while various light intensity had no significant effects. In the sperm release, light intensity with 100 μmol photons m-2 s-1 had the best condition on with a value of 29.33 ± 1.7 pcs / 6.72 × 104 μm2 in the second day. In addition, 25℃ temperature was the optimal condition on fertilization rate with 84.74 ± 6.59 % value. Conversely, light intensity had no significant impact. All tested temperatures (18, 22 and 26℃) were best at 100 µmol photons m-2 s-1 with a survival rate value of 74.33 ± 4.51, 1.67 ± 0.58, and 5.33 ± 6.11 % and growth performance of 5.58 ± 0.25, 3.43 ± 0.17, 3.61 ± 0.08 mm, respectively. The best binding rate was observed in 15℃ temperature cultured in the filter, cotton rope and nylon rope was 86.33 ± 2.52、84.33 ± 1.53 and 82.00 ± 2.00 % respectively. Light intensity showed no significant differences. In the different light intensity, 20 μmol photons m-2 s-1 cultured in the filter and cotton rope was found best with 89.00 ± 1.00 and 82.67 ± 1.53 % values, respectively. In terms of growth performance, maximal value was found in 50 μmol photons m-2 s-1, cultured on canvas and nylon rope under 25℃ about 2.53 ± 0.05 and 2.44 ± 0.06 mm respectively. On the other hand, 20℃, cultured at canvass with 100 μmol photons m-2 s-1 had the optimal condition for growth performance about 2.34 ± 0.07 mm. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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