Genomic analysis of serologically untypable human enteroviruses in Taiwan
| Autor: | Yeh Sheng Chien, 錢葉盛 |
|---|---|
| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 Enteroviruses (EV) are single-stranded, positive-sense RNA viruses in the Enterovirus genus of the Picornaviridae family. All enteroviruses have a similar genomic organization (7.2–8.5kb). The capsid proteins are coded on the 5’end of the ssRNA in a section called P1 (precursor 1). The nonstructural proteins are coded on the remaining sections of the genome, which are called P2 and P3. Changes in the structural protein genes of different enterovirus species reflect phylogenetic relationships. EV cause various clinical manifestations, including cutaneous, visceral, and neurological diseases. The Enterovirus genus consists of 12 species, including Enterovirus A (EV-A, 25 serotypes), Enterovirus B (EV-B, 63 serotypes), Enterovirus C (EV-C, 23 serotypes), Enterovirus D (EV-D, 5 serotypes), Enterovirus E (EV-E, 4 serotypes), Enterovirus F (EV-F, 6 serotypes), Enterovirus G (EV-G, 11 serotypes), Enterovirus H (EV-H, 1 serotype), Enterovirus J (EV-J, 6 serotypes), Rhinovirus A (80 serotypes), Rhinovirus B (32 serotypes), and Rhinovirus C (55 serotypes) Chapter Two indicated that we successfully established a NGS platform to conduct genome sequencing for the serologically-untypable enterovirus isolates. Among 130 serologically-untypable isolates, 121(93%) of them were classified into 29 serotypes using CODEHOP (COnsensus-DEgenerate Hybrid Oligonucleotide Primer)-based RT-PCR to amplify VP1 genes (VP1-CODEHOP). We further selected 52 samples for NGS and identified 59 genome sequences from 51 samples, including 8 samples containing two virus genomes. We also detected 23 genome variants (nucleotide identity |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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