Dielectric Barrier Discharge Based Remote Atmospheric Pressure Plasma Deposition for One-step Protein Immobilization

Autor: Yang, Chu-Hao, 楊筑皓
Rok vydání: 2018
Druh dokumentu: 學位論文 ; thesis
Popis: 107
In recent years, atmospheric pressure plasma (APP) has been widely used for bio-medical applications. APP can achieve non-thermal and mild discharge, reducing the heat influence to bio molecules. Without using vacuum systems, the cost of APP system can become lower. In this study, a dielectric-barrier-discharge based remote APP deposition (DBD RAPPD) system is used for protein immobilization. Since in APP system the deposition is occurred in the discharge region, protein may be denatured or damaged by the factors in the plasma, like heat, reactive species and UV. The deposition region is limited by area of the electrode in APP system. With remote plasma, deposition can occur at the downstream region of the plasma. Influence of plasma on protein can be reduce, large surface treatment and uniform coating can be achieve by this DBD RAPP system. The DBD RAPP system used two parallel plate DBD, which has a 1mm distance between them. Aluminum tapes (50mm x 50 mm) are used as the electrodes. The working gas in plasma is helium, and the precursor is ethylene. The protein we used is BSA (Bovine serum albumin). Helium goes through the atomizer to generate protein aerosol. Ethylene reacts with plasma and becomes plasma-polymerized ethylene (ppE) then deposited onto the substrate. Upstream mixing (mixing BSA aerosol and ethylene before gas going through the discharge region) and downstream mixing (mixing BSA aerosol and ppE at the downstream region outside the discharge) are used for experiments discussions. BSA is immobilized by entrapment of ppE or bonded with functional groups. FTIR was used for analyze the composite of coatings. SEM was used for investigate the morphology of coating surfaces. The coatings were rinsed by micro fluid to detect BSA adhesion. Surface profiler was used to analyze the thickness of coatings. Immunostaining was used for detecting the protein activity. In the result the signal and structure of BSA-ppE coating are detected and remained after rinse. Some appropriate experiment parameters for the immobilization are found.
Databáze: Networked Digital Library of Theses & Dissertations
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