The Study of Interaction Between Novel Bio-materials and Human Cells
| Autor: | Shih, Nai-Chia, 施乃嘉 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 Despite of recent advances in the development of biomedical technology, prevalence of chronic diseases remains high in many countries. Not only it is a huge financial burden in many countries’ healthcare systems, chronic diseases also create emotional toll and affect quality of life of patients and their family. Therefore, there is an urgent need to continue to build on the current understanding and further improve current treatments for chronic diseases. Two different chronic diseases were investigated in this thesis, and that two different novel technologies were developed. The first chronic disease that was investigated was pressure ulcer. The disease is common to patients that are bedridden or confined to a wheelchair, in which localized damage occur in the skin as a result of pressure. If the wound is not closed, the disease can result in serious infections and kidney failure. Therefore, to promote wound closure, a temperature sensitive gel-patch system in harboring drug-loaded liposomes was developed and released them at body temperature. Since oxidative stress is a major cause of progression of pressure ulcer, tempamine (TMN), an antioxidant, was formulated into a liposomal drug (Lipo-TMN). Subsequently, Lipo-TMN were embedded in PLA-Gelatin system, in which the liposomal drugs could only be successfully released at 37C. Since epithelialization and angiogenesis are the two major events that need to happen for a successful skin wound closure, the therapeutic efficacy of the released Lipo-TMN were investigated in human epithelial cells (HaCaT) and human endothelial cells (HUVECs) that were treated with hydrogen peroxide (H2O2). Better cell viability was noticed in cells that received Lipo-TMN that embedded in PLA-Gelatin compared to the cells received TMN embedded in PLA-Gelatin, suggesting liposomes were required to sustain the anti-oxidant activity of TMN.. Expressions of apopotic genes and proteins were significantly downregulated in cells treated with Lipo-TMN embedded in PLA-Gelatin. Collectively, the data indicated the PLA-Gelatin system was effective in releasing Lipo-TMN at 37C and the released Lipo-TMN were able to alleviate the oxidative damages in human epithelial and endothelial cells. The second part of the thesis was to develop a novel 3D printing ink formulation that could be used to fabricate custom-designed arteriovenous graft (AVG) for patients with end renal stage disease (ESRD) that requires hemodialysis. Since platelet adhesion is a major factor that resulted in dysfunctional AVG, which can cause mortality if not address immediately, therefore it is important the newly developed 3D printing ink formulation contain anti-adhesive properties. Several anti-adhesive materials were screened for their ability to be 3D printed. To ensure the materials could indeed prevent plasma protein adsorption and platelet adhesion, the 3D printed AVGs were submerged in human platelet rich plasma (PRP), and the presence of fibrinogen and platelets were immunostained. It is believe this novel formulation will be useful in the future development of a custom-designed AVG for ESRD patients, which will promote a better quality of life as they don’t have to have their implanted AVGs constantly replace because of restenosis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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