Insecticidal effect of Metarhizium anisopliae on five mosquito species
| Autor: | Bo-Kai Chen, 陳柏凱 |
|---|---|
| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 The aim of these study is to evaluate the virulence of M. anisopliae against mosquito larvae and pupae of five species, including Aedes aegypti (Linnaeus), Aedes albopictus (Linnaeus), Culex pipiens molestus Forskal, Culex tritaeniorhynchus Giles, and Armigeres Subalbatus (Coquillett). The data revealed that M. anisopliae exhibited lethal effects on mosquito larvae and pupae and the 1st instar larvae have the highest susceptibility in all five species. In addition, it also indicated that the value of LC50 of five species depended on larval stage. When pupae infected by M. anisopliae, the pupae could not successfully emerge as adults, and, pupae showed the lower susceptibility of all five species when compared with larvae. When comparing the susceptibility of five mosquito species to M. anisopliae, both Culex species had the highest susceptibility to M. anisopliae, and followed by two Aedes species and Ar. subalbatus. In a simulated test of M. anisopliae conidia suspension to the Ae. aegypti, it showed that the mortality rate of M. anisopliae against larvae after 5-day outdoor treatment, the infection rate was significantly lower than the control. However, the treatments in a growth chamber has a significant difference 6-day after treatment. In a study of temperature effect on M. anisopliae infecting Ae. aegypti larvae, the data shown that mosquito larval infectivity of M. anisopliae was increased with the temperatures increasing, and the highest infectivity was at 31°C. The results showed that the 18S gene of M. anisopliae was detected inside Ae. aegypti larvae body after infection by M. anisopliae 12 hours after. Being an agriculture environmentally friendly microbial pesticide, these studies have confirmed that M. anisopliae could kill mosquito larva and pupa effectively and could be considered as a potential biopesticide candidate for the mosquitoes in the future. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|