Establishment of multiplication protocols on themedicinal plant Glycine tomentella Hayata
| Autor: | Shu-Ju Liu, 劉淑如 |
|---|---|
| Rok vydání: | 2019 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 In this study, the proliferation system of the medicinal plant of Glycine tomentella Hayata (one root of kinmen) was established by the micropropagation methods, including seed germination methods, establishments of sterile seedlings, inductions of shoots and adventitious roots from explants, comparisons of B5 and MS media for shoot multiplications, and acclimation and transplanting of seedlings in soils. The seed germination test results showed that the germination rate of peat soil sown in the B5 solution with half-amount of macroelements and 1.5% sucrose was 52.2%, whereas the germination rate in the same media without sucrose was 45.6%, suggesting additions of sucrose may enhance seed germination rates of Glycine tomentella Hayata. Both MS and B5 media were tested for shoot multiplications. Results showed that when explants grown on MS media in comparison to explants grown on B5 media for 12 weeks, plant heights were shorter, numbers of cluster buds from explants were fewer, leaves were light green, thin, and large. Additionally, when explants grown on B5 media with 0.4 ppm BA and 0.04 ppm NAA compared to explants grown on MS media with same concentration of BA/NAA for 12 weeks, plant grew more vigorously, cluster bud numbers from explants were more, leaves were greener and smaller, indicating B5 media with BA and NAA were more suitable for shoot multiplications. Additions of plant growth regulators and locations of buds were examined to determine their effects on shoot multiplications of the Glycine tomentella Hayata explants. Results showed that when explants grown on B5 media with 0.1/0.01 ppm BA/NAA and 0.2/0.02 ppm BA/NAA had higher numbers of shoots than other tested media. The average number of shoots per explant was 8.3 when explants grown on B5 media with BA and NAA for 12 weeks. The rooting rate of the Glycine tomentella Hayata explants was 97.8% when grown on B5 media with 0.2-0.8 ppm IBA for 12 weeks. When grown on B5 media with 0.4 and 0.8 ppm IBA for 12 weeks, root numbers per explant were 13.8 and 12.4 which were higher than other tested media. Rooting rate of explants was 95.6% when grown on B5 media with 0.4 and 0.6 ppm NAA after 12 weeks. Root numbers per explant were 19.4 and 18.6 on these two media that were higher than other tested media. The bud was inverted to examine its effect on rooting of the Glycine tomentella Hayata explants. Results showed no roots were obtained when explants grown on B5 media iii with or without plant growth regulators. The Glycine tomentella Hayata seedlings were transplanted in soil for 30 days, survival rates of seedlings were 100%, suggesting optimal tissue culture protocols for the Glycine tomentella Hayata sterile seedling proliferations were successful established in this study. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|