Application of magnetic graphene oxide for the immobilization of lipase.
| Autor: | Jun-Kai Huang, 黃俊凱 |
|---|---|
| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 107 In this study, the large specific surface area of graphene oxide and the superparamagnetic of nano Fe3O4 were used to synthesize the magnetic graphene oxide composite as a carrier. We immobilized the lipase from Candida rugosa Type VII on the carrier and make of series of operational stable test. The graphene oxide(GO) was synthesized by Hummers method. The graphene oxide was investigated by FTIR,XPS,TEM and XRD. The nano Fe3O4 was prepared by thermal decomposition method, and we analysis nano Fe3O4 with FTIR,TEM. Then we utilize EDC/NHS to synthesize magnetic graphene oxide composite, and FTIR,TEM were employed to characterize the structure and morphology. We immobilize lipase on magnetic graphene oxide and make of series of operational stable test. It is worth noting that are thermal and solvent tolerance test. The immobilized lipase residual activity at 80 °C is still higher than the free by 43.7 % (free state 14.79 %, immobilized state 58.5 %).The relative activity of the immobilized state in the solvent resistance test is much higher than that of the free state, especially 82.3% higher relative activity in n-hexane. Based on the above results, we proved immobilized lipase on magnetic graphene oxide can effectively improve the problem of heat and solvent resistance of the enzyme. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|