Monascus and Trigonella foenum-graecum water extracted mixture alleviates high fat diet-induced non alcoholic fatty liver in mice

Autor: HUANG, LI-NING, 黃俐寧
Rok vydání: 2019
Druh dokumentu: 學位論文 ; thesis
Popis: 107
Nonalcoholic fatty liver disease (NAFLD), a spectrum of diseases with fat accumulation-induced liver damage, has become an important global issue. Studies showed that NAFLD is closely related to obesity, type 2 diabetes mellitus and cardiovascular disease.With excess fat accumulation, the hepatocytes develop insulin resistance followed by increased free fatty acids, oxidative stress, and steatosis, which subsequently result in the progression of NAFLD from simple steatosis to nonalcoholic steatohepatitis, and further to liver fibrosis, cirrhosis, and hepatocellular carcinoma. Monascus and fenugreek (Trigonella foenum graecum) have been demonstrated to have beneficial effects on reducing hyperlipidemia, improving insulin resistance and decreasing oxidative stress. Therefore, the aim of this study was to investigate the effects of Monascus and Trigonella foenum-graecum water extracted mixture alleviates high fat diet-induced nonalcoholic fatty liver in mice. Male C57BL/6J mice were intragastrically administered with deionized water (the NC and HF groups) or with a low, medium or high dose (the WL, WM, or WH groups) of Monascus and fenugreek water extracted mixture (MFW). After treated for 1 week, mice were started to pair-fed with a standard chow diet (13% calories from fat) for the NC group and a high-fat diet (60% calories from fat) for the other 4 groups. After 18 weeks of feeding, the results showed that the WL and WM MFW group had significantly decreased high fat diet-induced serum levels of triglyceride (TG), non‐esterified fatty acids (NEFA), alanine aminotransferase (ALT) activity and liver TG content. The WM and WH MFW group further had significantly decreased high fat diet-induced serum levels of cholesterol, liver cholesterol and NEFA content. But, only the WM MFW group had significantly decreased lipid accumulation status and the protein expression of phosphate nuclear factor kappa-light-chain-enhancer of activated B cell (p-NF-κB) of inflammatory responses in liver. However, the WL MFW group had significantly increased the protein expression of acetyl CoA carboxylase (ACC) of lipogenic enzymes; the WL, WM and WH MFW group had no significantly difference in high fat diet-induced imbalance of lipogenic and lipolysis enzymes, activation of AMP-activated protein kinase (AMPK) is a crucial cellular energy sensor and apoptotic proteins. In summary, Monascus and Trigonella foenum-graecum water extracted mixture may via decreased lipid accumulation status and liver inflammation to alleviate high fat diet-induced nonalcoholic fatty liver.
Databáze: Networked Digital Library of Theses & Dissertations
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