COUP-TFII is required for morphogenesis of the neural crest-derived tympanic ring
| Autor: | Wen-Hsin Hsu, 許文馨 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Chicken Ovalbumin Upstream Promoter-Transcription Factor II (COUP-TFII) plays pivotal roles in cell growth, cell differentiation, and cell fate determination. Although genome-wide studies have identified COUP-TFII binding on gene sets mainly involved in neural crest cell (NCC) development and craniofacial morphogenesis, the direct functional connection between COUP-TFII and NCCs in vivo has not been well characterized. In this study, we show that COUP-TFII is expressed in a subpopulation of NCCs and their derivatives. To delineate the role of COUP-TFII in NCC development, Wnt1-Cre transgenic mouse was chosen to inactivate COUP-TFII in premigratory NCCs. Dramatically shortened tympanic ring with a bifurcated end was observed in Wnt1-Cre/+;COUP-TFIIflox/flox mutant mice with complete penetrance. Tympanic ring is a C-shaped intramembranous bone that provides physical support to the tympanic membrane. Even though the caudal part of external acoustic meatus invagination is misled, formation of the manubrium of the malleus (MM) in Wnt1-Cre/+;COUP-TFIIflox/flox mice is not perturbed, suggesting that the rostral half of the tympanic ring is sufficient to support proper MM development. Comparative analyses further revealed that loss of COUP-TFII in NCC lineage up-regulates the expression of Sox9 in the tympanic ring primordium and affects the distribution of preosteoblasts before mesenchymal condensation, uncovering an essential role of COUP-TFII in the patterning of tympanic ring development. Therefore, our study provides new insights into the role of COUP-TFII in NCC-derived intramembranous bone formation. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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