Investigating the Impacts of Conjugated Linoleic Acid on the Expressions of PPARs Signals in the Brain and Anti-anxiety Behaviour in Vivo
| Autor: | Ann Ying-An Chen, 陳映安 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Conjugated linoleic acid (CLA) refers to a group of positional and geometric isomers of linoleic acid with a conjugated double bond, and has shown to exert several effective physiological functions such as anticarcinogenic, antiobese, antidiabetic and antihypertensive properties. Most of its biological actions are attributable to cis-9, tans-11 and trans-10, cis-12 isomers. CLA has shown to act as potent ligands for peroxisome proliferator activated receptors (PPARs). PPARs are ligand-inducible transcription factors that belong to nuclear hormone receptors (NHR). In the central nervous system, PPARs are related to lipid metabolism, neural cell differentiation and death as well as inflammation and neurodegeneration. CLA activity in the brain may have a positive impact on neurological disorders, where an increase in inflammatory response seems to contribute heavily to their pathogenesis. Yet very little is known about the expressions of PPARs in the brain. The detection of PPARs signals in the brain and whether or not CLA exerts anti-anxiety properties will be determined in this study. Twenty mice were used in this experiment; they were randomized into four groups and housed in a temperature-controlled room under a 12-h-light/12-h-dark cycle. The control group was fed a basal diet and the other 3 groups received a high-energy (HE) diet for 28-days. Among them, 2 groups were treated with CLA1 (9c, 11t) or CLA2 (10t, 12c) supplementation along with a HE diet for 28-days. The following day behavioural examination was conducted with an elevated plus maze. The mice were scarified; brain and liver tissue were harvested for the detection of PPARs signals via real-time PCR. The results showed that neither CLA isomers had an impact on body weight gain. The Elevated plus maze showed a significant increase in the time spent in open arms in the HE and CLA2 groups, and arm entries showed a significant increase in HE group, whilst all comparing to the control group. Significant expression of PPARα in the liver, cerebrum, cerebellum, and hippocampus was found in both CLA groups. Significant upregulation was found in expressions of PPARγ in the liver in CLA1 groups, and also in CLA2 groups in the cerebellum and hippocampus. This study concluded that both CLA isomers had an impact on PPARs expressions in the liver and brain. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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