Simultaneous Determination of Basic Drug Enantiomers in Human Urine and Serum Samples Using Capillary Electrophoresis Combined with Dispersive Liquid-Liquid Microextraction
| Autor: | HSU, SHU-HUI, 許淑惠 |
|---|---|
| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 This study was divided into two parts. First, rapid enantioseparation of three basic drugs (RS-Carvedilol, RS-Terbutaline, and RS-Metaproterenol ) was performed, with dimethyl-β-cyclodextrin (DM-β-CD) as the chiral selector and poly(diallyldimethylammonium chloride) (PDDAC) in the background electrolyte as buffer additive. Under optimized conditions, the separation system－which comprised 5mM DM-β-CD and 0.9 % PDDAC at pH 2.5, with a capillary length of 35 cm, enabled three pairs of RS-Carvedilol, RS-Terbutaline, and RS-Metaproterenol and internal standard (losartan potassium) to be successfully separated in 10 min. Second, dispersive liquid－liquid microextraction was used in capillary electrophoresis. Under optimum conditions,the use of 1.00 mL of sample solution, 30 μL of dichloromethane as the extraction solvent, and 200 μL of acetonitrile as the dispersive solvent and, yielded a high enrichment factor in the range of 121－124 at an extraction time of less than 3 min. Moreover, a strong linearity was obtained from 0.09－to 10 μM (r >0.9956). In real samples, the recoveries of RS-Carvedilol from urine and serum samples were in the ranges of 74 %－123 % and 70 %－127 %, respectively. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|