Effect of Abscisic Acid on Healing Physiology of Tomato Grafted Seedling
| Autor: | WANG, PING-YUAN, 王品元 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 This research aimed to study the effects of foliar application with abscisic acid (ABA) to the tomato grafted seedlings to reduce physiological stress during the healing period. In this test, tomato ‘Yu Nu’ was used as scion and eggplant ‘EG203’ was used as stocks. Grafted seedlings were applied with 0 (control), 500, 1000 and 1500 mg L-1 ABA, respectively, and then placed in the accumulation chamber. Record physiological changes of tomato grafted seedling within 14 days after grafting. The results showed that foliar application with ABA were reducing the tomato grafted seedlings withering immediately and improve the graft survival rate significantly. The structural development of the graft union formation in tomato plants is studied. Longitudinal section diagrams from graft union showing graft gap at the 1st-3rd day after grafting. Longitudinal section of the graft union showing the cell division situation is obvious at the 5th-7th day after grafting. Longitudinal section of the graft union showing that tissue of stock and scion mixed each other, parts of graft union were connected at the 9th-13th day after grafting. The results of scion and rootstock xylem bundles stained with blue flower dye value showed that the grafted seedling of 500 mg L-1 ABA treatment had significantly higher at the 13th day after grafting. The results of functional vascular connections showed that the grafted seedling of 1000 mg L-1 ABA treatment had significantly smaller gap of the sugar content between scion and rootstock at the 13th day after grafting. The content of proline in the control group was significantly higher than treatment group at initial stage after grafting. The results of superoxide dismutase, peroxidase, and ascorbate peroxidase activity showed that control, 500, 1000 mg L-1 ABA treatment reached the highest value at the 7th day after grafting. The results of chlorophyll fluorescence parameter showed that all treatment had obviously lower Fv/Fm at initial stage after grafting. At 9th day, Fv/Fm recovered gradually. The results showed that the foliar application with ABA at appropriate concentrations reduced the tomato grafted seedlings withered ratio, proline content, and improve the graft survival rate significantly. In addition, 500, 1000 mg L-1 ABA treatment had better performance of promote nutrient transport. The results of this research can be improved the quality of grafted seedlings with positive benefits. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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