To establish reproducible chronic constriction injury in rat sciatic nerve: conformational changes and functional loss

Autor: Szu-HanChen, 陳思翰
Rok vydání: 2018
Druh dokumentu: 學位論文 ; thesis
Popis: 106
Surgical nerve decompression for compressive neuropathy is the gold standard procedure and most patients have satisfied outcomes for symptom relief. However, the patients still suffered from neuropathic pain resulting from new, residual or recurrent compression neuropathy after surgeries. The severity of mechanical compression plays an important role for peripheral neuropathy. By reviewing previous animal models published for compressive neuropathy, there were no reproducible results in terms of tension-controlled. Therefore, we established a modified animal model of tension-controlled compressive neuropathy in rat sciatic nerve in order to analyze resultant functional outcomes. We established a modified animal model for compressive neuropathy under different tension stretch and then evaluate the behavioral function of injured sciatic nerve. The muscle atrophy and nerve degeneration were measured by histological assessments. We would also survey the neuro-inflammation and vascular disturbance in the regenerated nerve by using immunohistochemical (IHC) staining. We observed peripheral compressive neuropathy made behavior exchanged on the affected leg of rat including sensory dysfunction and muscle atrophy. If the mechanical force compressed stronger, nerve degeneration and muscle weakness made more severe. Those behavior results in the new animal model of peripheral compressive neuropathy were similar to clinical findings in humans. In the histological findings, we also found the mechanical compression proportional to nerve degeneration and neuroinflammation. In the future, we will confirm the new mechanism-based treatment by surgery or medication on the animal model to provide more efficient outcomes with lower side effect.
Databáze: Networked Digital Library of Theses & Dissertations
Externí odkaz: