Application of harpin proteins on alpine tea tree seedlings growing in the greenhouse condition
| Autor: | Chun-Kai Huang, 黃俊凱 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Tea is one of the most widely consumed beverage in the world. As the growing demand for tea in Taiwan, alpine tea plantations production is insufficient supply. Tea tree (Camellia sinensis) is suitable for growing in high-altitude mountainous areas. Due to limited area of mountain reclamation, to grow alpine tea tree in lowland areas will be a promising project, but the heat damage will cause the browning of the leaf margin and dehydration of the tea tree, it is one of the main problems of grow alpine tea tree in lowland areas. The harpin proteins produced by phytobacteria are secreted into plant apoplast via type III secretion system (T3SS) and thereby elicit the hypersensitive response (HR). Several studies showed that harpin proteins can promote plant growth, enhance stress tolerance, pathogen resistance and induces pathogenesis-related (PR) gene expression. In this study, harpin proteins HrpWAaa, HrpYAaa, HrpZPav and HopAK1Pav were overexpressed in Escherichia coli using T7 RNA-polymerase- dependent system and then were partial purified by boiling. Subsequently, the tea tree seedlings growing on lowland were sprayed with different concentrations of harpin proteins. Interestingly, the tea tree seedlings treated with harpin proteins showed higher survival rate, antioxidant system activity, Thaumatin-like protein (TLP) gene expression and more cell death in the inoculated leaf. In addition, based on the high performance liquid chromatography (HPLC) analysis, the phenolic compounds including gallic acid (GA), caffeine, epicatechin-3-gallate (ECG), epigallocatechin-3-gallate (EGCG) and teaghrelin (TG) in the leaves were elevated when the tea tree seedlings were treated with harpin proteins. Taken together, the results revealed that the harpin proteins are able to enhance resistance to heat for tea tree seedlings growing in the lowland environment and affect the production of secondary metabolites. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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