A sandwich-type electrochemiluminescence immunosensor based on graphene quantum dots for the detection of carcinoembryonic antigen
| Autor: | Shao-Hui Pan, 盤劭慧 |
|---|---|
| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 In this study, we designed a simple and sensitive sandwich-type electrochemiluminescence (ECL) immunosensor based on GQDs labelled secondary antibody (GQDs-Ab2) as a bioprobe to detect the concentration of the tumor marker carcinoembryonic antigen (CEA) on a GQD-Au NPs modified glassy carbon electrode (GQD-Au NPs/GCE). GQDs with excellent light stability and high specific surface area provided an efficient biointerface to control the loading amount of Ab2. First, the primary antibody, anti-CEA (Ab1) was immobilized on the GQD-AuNPs modified electrode by covalent binding, then the antigen and GQDs-Ab2 were successfully immobilized by specific interactions to form a sandwich-type immunocomplex. The principle of ECL detection was that the ECL intensity will be increase by the ECL reaction between GQDs and K2S2O8 after binding GQDs-Ab2 to CEA. The prepared ECL immunosensor showed high sensitivity and wide liner for detection CEA in the range of 0.005 - 50 ng/mL and the limit detection was 2 pg/mL. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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