Novel Aggregation-Induced Emission Fluorophores Applied on Cellular Studies
| Autor: | Jyun-Wei Chen, 陳俊瑋 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 In this study, we focused on developing the newly organic molecules on the therapy and diagnosis of cancers. In the experiments, we synthesized some simple fluorescent organic molecules and enhanced their performances in the cells by the unique properties of Aggregation-Induced Emission (AIE). First, we synthesized an asymmetric 1, 6-disubstituted naphthalene (NV-12P), which can generate particular reactive oxygen species to undergo type I photodynamic therapy under irradiation. Furthermore, this compound can specifically localize in mitochondria in cancer cells to exhibit selective dark cytotoxicity, and exhibit efficient photodamage in cancer cells due to intracellular bright spots. Results suggested that this compound can be a potential dual-toxic efficacy molecule. Additionally, a water-soluble pH sensor ADA was designed and synthesized based on the molecular design of photo-induced electron transfer (PET) and intramolecular charge transfer (ICT). The fluorescent emission response against a pH value located in the range of 3~6, which is suitable to label intracellular pH dependent microenvironments. Finally, we not only used ADA to identify intracellular pH gradients between different cell lines, but also developed ADA as a fluorescent molecule that labeled normal cells by the unique fluorescence intensity of ADA in normal cells, making it effective as the control group at the time of cancer diagnosis. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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