Study on Preparation of Nanofiber Dye Affinity Membrane and the Adsorption Characteristics for Malate Dehydrogenase
| Autor: | CHIEN,SHIH-CHIEH, 簡士傑 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 In this study, the cellulose acetate (CA) nanofiber membrane was prepared by electrospinning technique. The operating parameters was to control the diameter including CA concentration, applied voltage, spinneret tip size and collector distance. The optimal conditions of electrospinning process for CA nanofiber membrane was found to be 25 kV (applied voltage), 12.5 mm (distance), and 0.016 mL/min (flow rate). The obtained CA nanofiber membrane was then treated with 0.1 M NaOH in H2O/ethanol (4:1) for 3-12 hr to obtain regenerated cellulose (RC) nanofiber membrane. The RC nanofiber membrane was further surface functionalized with reactive orange 4 dye, as a dye pseudo-affinity nanofiber membrane. To investigate the adsorption characteristics of dyed nanfiber membrane for malate dehydrogenase (MDH), some operating parameters were investigated, such as the hydrolysis time of RC nanofiber membrane, the immobilized density of dye, adsorption pH, and disrupted yeast concentration. The optimal dye immobilized density and adsorption pH were initial dye concentration of 5.0 mg/mL and pH value of 8.0, respectively. Finally, the elution efficiency for the adsorbed MDH by dyed membrane was investigated under various conditions, such as elution pH, salt concentration, additive reagent and its concentration. The preliminary results showed that the recovery and purification factor were found to be 51.39% and 30.10 folds, respectively with 1 M NaCl in 10% ethylene glycol at pH 5 acetate buffer. Hence, the dyed nanofiber member has potential for use in the purification of MDH from clarified yeast homogenate. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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