Effect of Ethanol Extract of Cocoa Shell on B16-F10 Melanocyte
| Autor: | Tzu-Yang Lin, 林子揚 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Theobroma cacao is one of the most economical crops and it is the main raw material for making chocolate and its by-products. In the past, cocoa was shown to have good pharmacological activity, including: treatment of anemia, mental fatigue, tuberculosis, gout, kidney stones, and resistance Cancer and the role of scavenging free radicals. However, the current study has not confirmed that the cocoa shell ethanol extract has the ability to tanning the skin. In this study, the Cocoa shell ethanol extract was tested with B16-F10 melanocytes and the melanin content, tyrosinase activity, and protein changes in the melanin synthesis pathway were observed. The results showed that the increase in the concentration of the ethanol extract of the cocoa shell resulted in increased melanin content and tyrosinase activity in B16-F10 melanocytes, and the use of immunostaining assays to investigate the pathway of melanin synthesis in the signal transduction pathway. As a result, it was found that the cocoa extract of the cocoa shell can activate melanogenesis-related proteins Tyrosinae, Microphthalmia-associated transcription factor (MITF), Tyrosinase, etc -related protein-1 (TRP-1), Dopachrome tautomerase (DCT) and other protein expressions. From the above results, it was deduced that the cocoa shell ethanol extract has increased melanin synthesis on the skin, and has considerable potential for the development of tanning products. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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