Study on the Molecular Mechanism of Trace Mineral Zinc on Articular Chondrocytes
| Autor: | Tzu-Ching Huang, 黃姿菁 |
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| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Osteoarthritis (OA) is a common chronic disease especially in the coming aging population society. It is characterized as increase of oxidative stress, decrease of articular cartilage extracellular matrix, chondrocyte death, and cartilage damage. Zinc is an essential trace element. It has been reported that zinc is associated with antioxidant ability. The deficiency of zinc may inhibit chondrocyte proliferation. The present study examined the potential of zinc as a preventive supplement against OA using the in vitro chondrosarcoma cell line SW1353 and an in vivo Wistar rat model to mimic OA progress induced by monosodium iodoacetate (MIA). The results demonstrated that, in SW1353 cells, 5 μM MIA exposure increased oxidative stress and decreased the expression of GPx1 and Mn-SOD but still increased GSH levels and HO-1 expression and enhanced the expression of interleukin (IL)-10, IL-1β, and matrix metalloproteinase (MMP)-13. Zinc addition could block these changes. Besides, the expression of Nrf2 and phosphorylated (p)-Akt was dramatically increased by zinc, implicating the p-Akt/Nrf2 pathway in the effects of zinc on MIA-treated cells. A rat model achieved similar results as those of cell culture, and 1.6 mg/kg/day of zinc supplementation is sufficient to prevent OA progress, while 8.0 mg/kg/day of zinc supplementation does not have a better effect. These findings indicate that zinc supplementation exerts a preventive effect with respect to MIA-induced OA progress. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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