The effects of quercetin and genistein on lipopolysaccharide-induced inflammatory responses in C2C12 skeletal muscle cells
| Autor: | Cai-Ying Shih, 施采瑩 |
|---|---|
| Rok vydání: | 2018 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 106 Inflammation response acts as a defense against foreign aggression, but chronic inflammation plays a close relationship in pathogenesis and progression of many diseases. It was reported that exposure of RAW 264.7 macrophages with lipopolysaccharide (LPS) could induce inflammation via activation of mitogen-activated protein kinase (MAPKs) signal transduction and nuclear factor kappa B (NF-κB) transcriptional activity to enhance expression of the pro-inflammatory factors and enzymes such as inducible nitric oxide synthase, cyclooxygenase-2, interleukin-6 (IL-6), interleukin-1β (IL-1β) and tumor necrosis factor-α (TNF-α). Many Phytochemicals such as quercetin and genistein via inhibiting MAPKs-NF-κB pathway, attenuate the LPS-induced inflammation. In addition to immunocytes, other body tissues like adipose and muscle tissues treated with LPS could produce inflammatory cytokines such as TNF-α, IL-1β and IL-6 which are associated with metabolic changes, elevation of blood lipids, gluconeogenesis, protein catabolism and insulin resistance. Quercetin and genistein occur ubiquitously in the human diet including fruits, vegetables and soybean and its products. It is reported that quercetin and genistein has healthy beneficial properties including anti-carcinogenesis, anti-inflammation and anti-oxidation. The aim of current study is to investigate the effects of quercetin and genistein on LPS induced inflammation in C2C12 skeletal muscle cells. Treated with LPS and quercetin (5 μM or 10 μM) or genistein (5 μM) for 18 hrs has no cytotoxic effect on C2C12 skeletal muscle cells determined by 3-(4,5-dimethylthiazol-2-yl)- 2,5- diphenyltetrazolium bromide assay. Quercetin and genistein significantly attenuated the LPS-induced mRNA expression of pre-inflammatory mediators, including IL-6、IL-1β、TNF-α. Additionally, quercetin and genistein inhibited LPS induced phosphorylation of extracellular-regulated protein kinase 2、inhibitor-kappa Bα and p65. Furthermore, treated with quercetin alone or combined with LPS significantly enhanced catalase, heme oxygenase-1 and quinone oxidoreductase 1 protein expression. In summary, quercetin and genistein decrease inflammation and increase antioxidant protein expression of in C2C12 skeletal muscle cells treated with LPS. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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