Mitochondria dynamic in the mouse brain infected by Angiostrongylus cantonensis
| Autor: | Cheng-Zhi Chen, 陳程志 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Angiostrongylus cantonensis, the rat lungworm, is the major cause of eosinophilic meningitis. Rats are the definitive host of this nematode. Human can be infected by eating the third larvae from intermediate hosts. The symptoms of eosinophilic meningitis are meningitis and eosinophilic pleocytosis. Mitochondrial dynamic is crucial for the regulation of cell homeostasis. In this study, we attempted to assess the direct effects of A. cantonensis infection on mice brain, and to explore the expression of mitochondrial morphology, dynamics, and function. There were 5 groups (normal and 1, 2, 3 and 4 week post infection) of mice (5 mice in each group and each mice was infected with 25 L3 of A. cantonensis). Fission of mitochondria can be observed by TEM (transmission electronic microscopy). The brain cells were severely damaged and the mitochondria of showed atrophy at third week post infection. By western blotting, the protein of mitochondrial fission, biogenesis and mitophagy was increased, and the fussion protein was decreased at second and third week post infection. The protein of apoptosis (caspase 8 and 9) had significant increasd. Tthe mouse meningeal after infection can be found inflammation and damage in the sections of the brain tissue. Larvae were embedded in the cortex, and cell infiltration could be found around larvae. In TUNEL assay, the number of apoptotic cells was increased in the mice brain at second week post infection, and brain cell necrosis was more serious at third and fourth week post infection. It was also found that brain cell necrosis was increased in infected mice by Annexin V test. For mitochondrial function, membrane potential was detected. It was increased after infection, but it could not be detected at fourth week post infection. This may due to cell necrosis and therefore membrane potential could not be detected. These results confirmed that the A. cantonensis infection can influence mitochondrial dynamic, biogenesis, morphology and function in mice model. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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