Functional Study of Pectin Methylesterases in Response to Abiotic Stress in Arabidopsis
| Autor: | Yin-Da, Wang, 王胤達 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Pectin methylesterase (PME) is considered a cell wall remodeling enzyme that catalyzes the demethylesterification of polygalacturonans, the major component in pectin. It has been demonstrated that PME involves in plant growth, development, and plant-pathogen interaction. In recent years, many literatures showed that PME can be induced by abiotic and biotic stress, although there are limited reports about heat stress (HS). In our previous studies, PME34, contributing to regulation of stomatal movement, was required for HS response in Arabidopsis. We showed that the function of PME34 could be regulated by ABA signaling pathway in acquired thermotolerance. In addition, PME12, PME53 and PME68 genes were specifically expressed in guard cells after ABA treatment and abiotic stresses by microarray database. Histochemical GUS staining showed exogenous ABA treatment could upregulate these PMEs expression in guard cells. PME12, PME53, and PME68 were regulated by salt, mannitol, and heat treatment. PME12, PME53, and PME68 were located to the apoplast and/or cell membrane. The activities of PME12, PME53, and PME68 increased after drought treatment. These pme mutants had no major difference in respiration rate, but the stomatal aperture of the pore width, width/length ratio, and density of the mutants and overexpression lines showed significant difference compared to the WT. Hence, PME12, PME53, and PME68 had important roles in making plants to adapt the drought stress. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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