Synthesis and Characterization of Protein Fibril-Containing Hybrid Materials
| Autor: | Min-Chih Chung, 鍾旻芝 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 For many years, amyloid fibrils have been considered the cause of amyloidosis. However, recent evidence has proved that amyloid fibrils are only the side product formed during amyloidogenesis. Moreover, amyloid fibrils belong to one kind of denatured proteins that have high stability and fiber structure. Therefore, the applications of amyloid fibrils are now receiving an increasing attention. Given their excellent mechanical property and stability, amyloid fibrils are suitable for material applications. In this research, attempts were made to first prepare the graphene oxide-amyloid fibril hybrid materials by adding hen lysozyme derived-amyloid fibrils into graphene oxide with different ratios, and then analyze their morphology, interaction, thermal and mechanical properties. The results show that during the synthesis, amyloid fibril and graphene oxide may both undergo disassembly: amyloid fibrils may disaggregate to form protofilaments, wherease graphene oxide may exfoliate. Furthermore, results suggest that the binding between protofilament and thinner graphene oxide may be governed by the electrostatic interaction and hydrogen bonding, which means the hybrid materials made of protofilament and thin graphene oxide exhibit lower energy. Comparison of the hydrid materials with different ratios indicates that the thermal stability could be enhanced upon adding more amyloid fibrils. However, as for the mechanical property, there exists a positive correlation between the rigidity and added amount of amyloid fibrils during the range of 1:0.016, followed by a decrease in the rigidity upon further increase in the amount of fibrillar species. We speculate that this may attributed to the formation of highly dispersed phase. In conclusion, the hybrid materials resulted from the addition of amyloid fibril to graphene oxide are expected to be more biodegradable and biocompatible with less toxicity. Also, the aforesaid hybrid materials exhibit adjustability. The thermal and mechanical properties could be controlled by the ratio between amyloid fibrils and graphene oxide. Therefore, these hybrid materials hold great potential for the applications in the fields of biomaterials and bio-sensoring. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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