Effect of Fucoidan on Anterior Cruciate Ligament Transection and Medial Meniscectomy induced Osteoarthritis in High Fat Diet induced Obese Rats
| Autor: | Alan Darmasaputra Ong, 王杰鴻 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Osteoarthritis (OA) has become one of the most common disabilities among elders, especially in female and those with metabolic diseases. The risk factors involved in OA include gender, obesity, previous injury, and heredity. Obesity-related OA is attributed to joint loading, cartilage disintegration, bone loss along with inflammation. Fucoidan is a sulfated polysaccharide extracted from brown algae. It has been studied for its antitumor, antiviral, anticoagulation and immunomodulation. In this study, we investigate the effect of fucoidan in surgery-induced OA on diet-induced obesity male rats. OA was induced by anterior cruciate ligament transection (ACLT) and medial meniscectomy (MMx) method. Male Sprague Dawley (SD) rats were fed 40% high fat diet for 4 weeks to induce obesity before ACLT+MMx to initiate OA. OA rats were administered intragastrically with water or fucoidan P.O. in three different concentration, 32 mg/kg, 64 mg/kg, and 320 mg/kg after surgeries for 40 days with high-fat diet in parallel. We observed that the swelling in the OA knee was alleviated and the hind paw weight distribution was rectified after feeding fucoidan, with significant effect on body weight and body fat reduction and plasma lipid were also analyzed. Our result indicated no significant variation on HDL-cholesterol, but reduced plasma triglycerides, total cholesterol, and LDL-cholesterol level. In addition, weight-bearing tests showed some improvement on fucoidan-fed groups. Our result suggests that the feeding of fucoidan may potentially improve meniscal/ligamentous injury of obesity induced OA. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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