Immune responses of recombinant VP1 antigens of serotype O Foot-and-Mouth Disease Virus
| Autor: | Lai, Yi-Chun, 賴怡君 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Foot and mouth disease (FMD) is a highly contagious viral disease of cloven-hoofed animals, and causing very huge economic losses. The infection of the virus induces vesicular. FMDV belongs to genus Aphthovirus within the family Piconaviridae. There are seven serotypes of FMDV. The O type virus is the main strain in Taiwan. There are VP1-VP4 which are the four structural proteins of FMDV. The VP1 carries important epitopes against FMDV inducing specific immune responses. The goal of this study was to recombinant FMDV VP1 gene into the pET24a vector, and used the Escherichia coli expression system to express this recombinant protein. After purified, the protein antigenicity was characterized by western blot and ELISA with anti-His and anti-FMDV VP1 to confirm the antigenic of recombinant protein. The VP1 protein was mixed with ISA206 adjuvant to produce subunit vaccine. The efficacy of recombinant protein vaccine was determined by antibody titer test. The results showed that the group of vaccine antibody titer higher than negative control. The immne result proved that the recombinant protein had immunogenic for FMDV, which suggest that the subunit vaccne may be a valuable vaccine candidate to prevent FMD on swine. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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