Effects of Matrine and Platelet-Rich Fibrin on Mouse Embryo Development
| Autor: | Su, Sheng-Fen, 蘇聖分 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Martine (Sophocarpidine ) is a nature alkaloid derived from Traditional Chinese Medicine-Sophora root (the root of sophoraflavescens Ait), and it has been documented Compendium of Materia Medica. Matrine has widely used in the viral infection and skin treatment. Recent studies have demonstrated that matrine induces apoptosis in cancer cells and inhibits the melanoma cell proliferation. The potential toxicity of matrine on mouse embryo development however is still unclear. In this study, blastocysts were cultured in the presence or absence of matrine (0, 25, 50 or 100 M ) for 24 hours. Results showed that matrine ( 25, 50 or 100 M ) has inhibition on the total cell number of blastocysts following 24 hour culture. This inhibition of matrine on embryonic cell growth was mediated by a selective effect of matrine on ICM cells without affecting on TE cells. Platelet-rich fibrin (PRF) is a second generation platelet concentrate prepared without anticoagulants. Extracts derived from PRF contain several growth factors, like platelet-derived growth factors (PDGF), insulin-like growth factors (IGF), vascular endothelium growth factor (VEGF) or transforming growth factors (TGF). To learn the potent of PRF whether can protect the embryonic cells from matrine inhibitory effect, we cultured blastocysts in the presence of matrine (100 M) with 10% of fetal bovine serum (FBS) or matrine with 10% of horse PRF extract for 24 hours. Results showed that PRF significantly increased the number of total embryonic cells compared with control group supplemented with FBS. In matrine treated blastocysts, the number of embryonic cells, either in the total or in the ICM cells has significantly improved by PRF. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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