Nanomaterials combining with artificial biomaterials for biosensor developments
| Autor: | Lee, Cheng-Yu, 李承宇 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 In this study, different biosensors combined with unique signal transfer and amplify strategies were presented. Each signal amplification strategy could be used in relative biosensor developments for various applications, which the performance, applicability and feasibility are demonstrated and confirmed in this study. Based on the major purpose to design platform analytical method, four brand new biosensors under the assistance of nanoparticles and biomaterials were developed. In first human IgE electrochemical biosensor, the human IgE is analyzed by aptamer modified gold nanoparticles coated electrode. With the amplification strategy of G-rich cDNA, the extreme low detection limit as 0.16 pM was achieved. The assay also showed good selectivity in the presence of interferences with 100 folds higher concentration than analyte. In the second part, an electrochemical biosensor combined with aptamer and rolling circular amplification for prostate-specific antigen (PSA) detection was developed. With the amplification of the RCA reaction and the isolation of functional magnetic nanoparticles, the biosensor showed good linearity from 100 fM to 10 nM with detection limit as 22.3 fM. Four interferences showed negligible effect with presented biosensor. The third biosensor was discussed about monitoring eosinophil cationic protein (ECP) during cell culture experiments. Heparin modified functional magnetic nanoparticles were used as ECP probe and magnetic drove for analyte gathering. With the pre-concentration and isolation effects provided by functional magnetic nanoparticles, ECP could be analyzed within concentration range from 1 to 1000 nM. The estimated detection limit was 0.30 nM based on the definition of signal to noise ratio. The biosensor also showed good recovery in the cell culture medium RPMI 1640 after incubated with Beas-2B cell line. The last aptamer based biosensor was researched for solving possible issues in LC-MS. Ethanolamine was used as model compound to demonstrate the proposed scheme, which is unsuitable to be direct analyzed by LC-MS. With the transference and amplification strategy by artificial designed barcode DNA, the ethanolamine was indirectly analyzed by LC-MS within linear range from 5 nM – 5 μM. The estimated detection limit was 1.2 nM. The developed biosensor provided a universal platform for other analytes by replacing aptamer and related barcode cDNA sequence. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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