Evaluation of micro/nano-materials for aflatoxicosis amelioration and anti-heat stress of Taiwan country chickens
| Autor: | Ching-Chin Chen, 陳清錦 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Aflatoxins are often found in the contaminated crops and feedstuff and thus aflatoxicosis seriously threatens the cost of livestock production. Clays and zeolitic materials are rich with aluminosilicates, which can bind to aflatoxins and thereby are used as a mycotoxin absorbent in feedstuff. The study was designed to examine the effect of micro/nano-clays as a mycotoxin adsorbent on the growth performance and to determine hepatic antioxidative capacity, TG content and inflammatory status and related to gene expression. 1-day-old red-feather Taiwan country chickens. were divided into 8 groups, control (CON), aflatoxin B1 (AFB1), AFB1+micro/nano-clay (TB, TBB1, or TBI1) with a high or low dosage (1000 and 200 ppm) for a feeding trial up to 11 weeks. In the feeding trial, birds were provided with feed containing 100 ppb AFB1 before age of 6 weeks, and then with 200 ppb AFB1 in the following 5 weeks. Besides, It were provided nano calcium carbonates with a high or low dosage (0.1 and 0.05%) in feed and water for a feeding trial up to 16 weeks. examined for their effects on anti-heat stress and egg quality in laying hens. AFB1 suppressed body weight gain and feed efficiency and promoted hepatic oxidative stress and inflammatory status in red-feather Taiwan country chickens. Dietary inclusion of TBI1 at the high dose increased body weight gain, feed intake and GSH content and TB inclusion suppressed hepatic GSSG content and interleukin-1 production, TBI1 inclusion reduced hepatic SMase (sphingomyelinase), ACC-1 (acetyl-CoA carboxylase 1), CPT-1 (carnitine palmitoyl transferase) transcript level, whereas dietary inclusion of any micro/nano-clays failed to ameliorate hepatic TG (triacylglycerol) and MDA (malondialdehyde) accumulation induced by AFB1. No noticeable changes in hepatic histology were observed by AFB1 and micro/nano-clays. In renal histology, the low dose of TBB1 and TBI1 inclusion improved vacuolization by AFB1. In laying hens, there was no statistically significant difference between water intake levels dietary inclusion of 0.1% nano calcium carbonate ameliorated rectal temperature and 0.1% inclusion in drinking water lowered blood pH value (P < 0.05). In egg quality, 0.1% inclusion in drinking water promoted egg shell strength and thickness whereas 0.05% inclusion promoted yolk weight (P < 0.05). In conclusion, micro/nano-clays effectively ameliorate aflatoxicosis and thus are potential as a commercial mycotoxin binder. Supplementation of nano calcium carbonate in drinking water can provide additional and immediate calcium source to replenish the loss of calcium during heat stress and thereby improve the quality of the eggshell. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
| Externí odkaz: |
|