Anti-coxsackievirus B3 activity and relevant mechanisms of glycyrol in Vero cells
| Autor: | Hsin-I Chou, 周欣儀 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Coxsackievirus B3 (CVB3), a member of the genus Enterovirus, is a positive single-strand RNA virus belonging to the Picornaviridae family. CVB3 mainly infects infants and children, and leads to acute myocarditis. In our previous study, glycyrol was isolated from Paris polyphylla Smith and showed activities of anti-enterovirus 71. This study continued investigating the anti-CVB3 mechanism of glycyrol in Afrian green monkey kidney (Vero) cells. The results showed that glycyrol had an anti-CVB3 activity of glycyrol with 1.44 ± 0.06 μg/mL of 50% inhibitory concentration (IC50). The four mechanisms of inhibiting CVB3 by glycyrol included a little bit prophylactic, slight inhibition of CVB3 adhesion to cells or virucidal, and excellent anti-viral replication effects. Glycyrol (3.00 μg/mL) significantly reduced the CVB3 progeny virus titer of 0.45-1.42 log PFU/mL and the VP1 expression in the CVB3-infected cells, in which the expression was 53% of that in CVB3-infected control. The results showed that glycyrol significantly inhibited the degradation of IκB⍺, the expression of NF-κB (p65), and the activation of mitogen-activated protein kinase (MAPK) (p38, p-JNK1/2/3 and p-ERK1/2) in the CVB3-infected cells. Additionally, COX-2, which is the downstream inflammatory factor, also decreased considerably. However, the endocytosis of coxsackievirus and adenovirus receptor (CAR) and the degradation of Ras GTPase activating protein (RasGAP) caused by CVB3-infection were not affected by glycyrol. In conclusion, possessing the ability to block the MAPK and NF-κB pathways, glycyrol had a great effect on inhibiting CVB3 via reducing the replication in CVB3-infected cells. Moreover, reducing the synthesis of COX-2 by blocking those pathways, glycyrol might have an ability to prevent from myocarditis by reducing inflammation. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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