Characterization of Agrobacterium Transformation Efficiency Affected by the Phytoplasma Effector SAP11.
| Autor: | Meng-Hua Tsai, 蔡孟樺 |
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| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Lacking cell wall and many essential genes required for biosynthesis and metabolic functions, phytoplasmas have become unculturable and phloem-limited plant pathogens. Disease symptoms caused by phytoplasmas including proliferation, little leaves, phyllody, virescence, leaf yellowing, hairy root, flower sterility and fruit deformity. Previous studies showed that the Aster Yellows Witches’ Broom (AYWB) phytoplasma contains 56 candidated effectors. Among them, the expression of secreted AYWB protein 11 (SAP11) in plants generated leaf crinkling and branching phenotypes. Our studies further demonstrated that SAP11AYWB homologues, SAP11CaPM and SAP11PnWB, can also cause the branching phenotype. Interestingly, during the generation of SAP11AYWB-transgenic Nicotiana benthamiana lines, we observed that SAP11 transgenic plants are easier to be obtained than vector only. This phenomenon suggests that SAP11 might affect the transformation efficiency. Here, using agroinfiltration, we demonstrate that SAP11 can increase the formation of callus and differentiation of shoots in N. benthamiana. Further more, in root tumorigenesis assay, we demonstrate that SAP11 can affect transformation efficiency by increasing the formation of tumors. Finally, in AGROBEST assay, we show that SAP11 can enhance the activity GUS reporter. This result suggest that SAP11 can also affect the transformation efficiency of other genes. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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