To investigate the effect of ferulic acid on formaldehyde and trans, trans-2,4-decadienal induced cellular toxicity in pulmonary epithelial cells
| Autor: | Ying-Jr Chen, 陳英智 |
|---|---|
| Rok vydání: | 2017 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Formaldehyde and trans, trans-2,4-decadienal (t,t-2,4-DDE) are two common chemicals in cooking oil fumes. Besides, formaldehyde is considered as an important indicator of indoor air quality. IARC classified formaldehyde as a group 1 carcinogen in 2012. This information reveals the importance of these two aldehydes in our daily life and their toxic effect on human being. Antioxidants play an important role preventing from diseases and improving human health nowadays. Ferulic acid is one of popular antioxidants having the abilities of anti-inflammation, scavenger of free radicals, and even anti-cancer. The aims of this study were to investigate cell viabilities of A549 human lung epithelium cells exposed to formaldehyde and trans, trans-2,4-decadienal using MTT assay and CCK-8 assay and to investigate the antioxidation effect of ferulic acid on the exposed A549 cells. In MTT assay, our results showed A549 cells exposed to formaldehyde concentrations greater than 5 mM for 1 to 4 h reduced cell viabilities. The cell viabilities reduced to 53% and 13%, when A549 cells exposed to 50 mM formaldehyde for 1 h and 4 h, respectively. Exposed to t,t-2,4-DDE concentrations ≥ 100 µM for 1h and those concentrations ≥ 50 µM for 2 to 4h also reduce cell viabilities. The cell viabilities reduced to 22% and 15%, when cells exposed to 400 µM t,t-2,4-DDE for 1h and 400 µM t,t-2,4-DDE for 4h, respectively. The same phenomena were shown in the results of CCK-8 assay. The results of ferulic acid antioxidation capability test showed ferulic acid treatment may increase cell viabilities of A549 cells. Exposed to 30 mM formaldehyde for 1h, cell viability increased 23%. As for t,t-2,4 DDE exposure, ferulic acid treatment slightly improved cell viabilities of A549 cells, in which the cell viability increased 12% for cell exposed to 200 µM t,t-2,4-DDE for 1h. The antioxidation effect of ferulic acid may not obvious while cells exposed to either high concentrations or long exposure time of formaldehyde and t,t-2,4-DDE. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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