The Effect of Thermosensitive Hydrogel Carrying Drugs on The Osteogenesis of Bone Marrow Mesenchymal Stem Cells

Autor: Jun-Hao Zhou, 周俊豪
Rok vydání: 2017
Druh dokumentu: 學位論文 ; thesis
Popis: 105
The major symptoms of osteoporosis are bone loss and decreasing bone mineral density, which eventually lead to bone fracture. The specific aims of the study were to evaluate the effect on osteogenesis of mesenchymal stem cells (MSCs) in two or three-dimensional culture environment with the supplements of dexamethasone (Dex), bisphosphonate drug Alendronate (Ale) or dendrimer poly(amidoamine) (Pamam) loaded with Alendronate (Pamam-Ale), and combined with hydroxyapatite (HA) and mPEG-PLGA thermoreversible hydrogel. The therapeutic effect in osteoporosis mice induced b glucocorticoid by using the combination treatment of MSCs, osteogenic inducing drugs and hydrogel was also observed. The results from osteogenic induction showed that 100 nM Dex,100 nM Ale and 10 nM Pamam-Ale could enhance alkaline phosphatase (ALP) activity and ALP and type I collagen gene expression. Highest level of ALP activity was found in the group of Pamam-Ale. When in combining with HA or hydrogel, highest level of ALP activity was found in the group of Ale and highest level of calcium deposition was seen in the group of Dex. The results from the femurs of osteoporosis mice injected with the combination of osteogenic inducing drugs, MSCs, HA and hydrogel found that no significant difference in the bone mineral densities of cortex bones between control and treatment groups. But the bone mineral densities of spongy bones from the treatment groups were higher than those of control group. Tissue sections showed that HA was distributed in the cortex bone and the porosity decreased in the spongy bone. In summary, the combination of osteogenic inducing drugs, MSCs, HA and hydrogel has the potential in osteoinduction and may be applied in bone defect repair.
Databáze: Networked Digital Library of Theses & Dissertations
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