The difference between Salmonella Paratyphi C- and Salmonella Choleraesuis-induced programmed cell death in macrophages
| Autor: | Yi An Chen, 陳奕安 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 105 Salmonella is Gram-negative bacteria and classified into two groups based on the disease patterns : typhoid serotypes and non-typhoid serotypes. Salmonella paratyphi C (SPC) is typhoid serotype and highly adapted to human which causes enteric fever. Salmonella Choleraesuis (SC) is non-typhoid serotype with broad host range and causes gastroenteritis. It is unknow why these two Salmonella with high genomic similarity make distinct clinical diseases. In this study, we aimed to compare the different level of inflammatory response and cell death mechanism in macrophages after SPC and SC infection. In our results, SPC induced higher cytotoxicity and IL-1β secretion than SC, and tended to induce pyroptosis mainly; however, SC tends to cause more apoptosis in macrophages. Besides, the cellular invasion and the expression of SPI-1 genes, such as sopB, sipB, hilA and prgI in SPC were higher than SC which grew in LB broth. SipB is a well know SPI-1 effector protein associated with Salmonella infection progress. We overexpressed SipB in SC and found that the strain showed higher infection ability and induced more cytotoxicity and IL-1β secretion in macrophages than wild type SC. In addition, SipB-overexpressed SC induced higher caspase-1activation and less caspase-3 activation than in macrophages wild-type SC. Taken together, differential expression of SipB between SPC and SC caused different level of immune response and cell death mechanism in macrophages. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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