Feasibility study on ectoine production of halophilic bacteria using Halomonas salina BCRC17875.
| Autor: | Tsai-Ling Chen, 陳柴綾 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 A natural cyclic amino acid derivative, ectoine acts as a protective substrate in many bacterial cells as a compatible solute. Synthesized and accumulated by halophilic and halotolerant microorganisms. Preliminary study of our group as well as those stated in literatures indicated that ectoine is an innovative, multi-functional natural compound not only stabilize proteins and other macromolecules, but also a with an effect in protecting Langerhans cells and UV-induced degradation of DNA. Comparison of ectoine concentration among the three strains obtained from Bioresource Collection and Research Center (BCRC) in Taiwan, the best strain for ectoine synthesis is Halomonas salina BCRC17875. Experimental results indicated that 4.5% appears to be the optimal concentration of NaCl, producing a maximum ectoine production of 2968 mg/L by Halomonas salina BCRC17875. Moreover, ectoine production was optimal in batch cultures when the temperature, pH, agitation rate was controlled at 30 ℃, pH 7.5 and 150 rpm, respectively. While the maximum ectoine concentration was increased by 11-folds when the glucose and glutamate both were 4%, the maximum crude ectoine production reached 2968 mg/L. Furthermore, the chemical structure and molecular masses of the purified ectoine were confirmed by nuclear magnetic resonance (1H-NMR) spectrometer and fast atom bombardment mass (FAB-MS) spectrometer, respectively. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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