The effect of extracts from Ampelopsis brevipedunculata for wound healing
| Autor: | Hsu Chieh Yun, 許婕筠 |
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| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Ampelopsis brevipedunculata, a chinese herbal medicine has anti-inflammatory and hemostatic ability. In this study, we extract the curing components by the reflux extraction of ethanol and analyze the effective components. Induced the inflammation of RAW264.7 cells by LPS and expressed some cytokines such as IL-1β, IL-6, TNF-α, and produce nitric oxide (NO), and then added the extracts of Ampelopsis brevipedunculata into medium to estimate the curing effect and found out the optimal adding concertation. In animal experiment, nanofibers of chitosan soaked in the extract of Ampelopsis brevipedunculata solution, and then covered on the wound position in the back of rat. To estimate the curing effect of extract Ampelopsis brevipedunculata, we observed the wound area recovery, organizational staining of H&E and Masson and the collage deposition. There is 91% wound recovery for the extract Ampelopsis brevipedunculata adding, and only 48% wound recovery for swab after 6 days curing. The full recovery of wound at 12 days by adding the extract Ampelopsis brevipedunculata, and the results of the organizational staining and collage deposition appeared the fast curing effect. To confirm the effect compositions of Ampelopsis brevipedunculata in wound curing and can be used to the clinical curing applications in further. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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