Studies on the Chemical Constituents and Biological Activites of Root Bark of Hernandia nymphaeifolia
| Autor: | Ya-Yun Hu, 胡雅筠 |
|---|---|
| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Hernanadia nymphaeifolia (Presl) Kubitzki (Hernandiaceae) is an evergreen tree, distributed in the tropical island shores of the Indian and western Pacific Oceans. In our studies on the anti-inflammatory constituents of Formosan plants, many species have been screened for in vitro anti-inflammatory activity, and H. nymphaeifolia was found to be one of the active species. In our search for compounds with anti-inflammatory activities, a new aporphine, N-formylhernagine (1), a new phthalimide, 5,6-dihydroxy- N-methylphthalimide (2), and 15 known compounds (3–17) were isolated and identified from the root bark of H. nymphaeifolia. Fifteen known compounds include two aporphines, oxohernagine (3) and hernandonine (4), three lignans, dehydrodeoxypodophyllotoxin (5), dehydropodophy- llotoxin (6), and podophyllotoxin (7), an amide, N-trans-feruloyl- methoxytyramine (8), a fatty acid, oleic acid (9), and eight steroids, mixture of β-sitosterol (10) and stigmasterol (11), mixture of β-sitostenone (12) and stigmasta-4,22-dien-3-one (13), mixture of stigmast-4-en-3,6- dione (14) and stigmasta-4,22-dien-3,6-dione (15), and mixture of 6β-hydroxystigmast-4-en-3-one (16) and 6β-hydroxystigmasta-4,22-dien- 3-one (17). The structures of all isolates were determined through spectral analyses and comparison of their physical and spectral data with literatures. Among the isolated compounds, N-formylhernagine (1), 5,6- dihydroxy-N-methylphthalimide (2), oxohernagine (3), hernandonine (4), N-trans-feruloylmethoxytyramine (8), and oleic acid (9) exhibited inhibition (IC50 values ≤ 6.23 g/mL) of superoxide anion generation by human neutrophils in response to formyl-L-methionyl-L-leuckyl-L- phenylalanine/cytochalasin B (fMLP/CB). In addition, N-formylhernagine (1), 5,6-dihydroxy-N-methylphthalimide (2), oxohernagine (3), N-trans- feruloylmethoxytyramine (8), and oleic acid (9) inhibited fMLP/CB- induced elastase release with IC50 values ≤ 7.41 g/mL. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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