Evaluation of three different herb extracts against grouper iridovirus infection
| Autor: | Võ Bích Xoàn, 武碧璿 |
|---|---|
| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Groupers are known to be an economically valuable aquaculture species in Southeast Asian countries. Groupers however, are known to be prone to viral infections that have been the root cause of great economic loss for aquaculture industries. Grouper iridovirus in Taiwan, is a major pathogen in marine fish aquaculture. This viral disease not only affect fry and juvenile grouper but are also known cause deaths in market-sized grouper, which are known to be a high priced, high demand cash crop in tropical marine culture. Herbal extracts as well as more traditional immuno stimulants could be applied in fish culture to replace antibiotic and chemotherapeutic agents. In this study, examination of three Chinese herbal extracts (Houttuynia cordata, Polygonum cuspidatum and Astragalus spp.) applied on GK cells. The experiment was carried out to evaluate the effects of three herbs on GK cells to against GIV. In this experiment, GK cells infection by iridovirus was replaced at 0µg/ml (control), 1 µg/ml, 5µg/ml and 10µg/ml of two herbs (Houttuynia cordata and Polygonum cuspidatum) at 0µg/ml (control), 1 µg/ml and 5 µg/ml in treatment of Astragalus spp. in pre-treatment and co-treatment. In other experiment, GK cells were evaluated with nine gene expression of IgD, IgM, MHC-II, IL-1β, Mx, TNF-α, MHC-I, C3 and IL-6 at different dose of herbal extracts. The result showed that the virus was still detected in co-treatment GK cells after 72 hours treated with three herbal extracts while in pre-treatment, the virus was not detected in all samples of this treatment. In gene expression experiment, IgD, IgM, MHC-II, IL-1β, and Mx were detected by using PCR method in treatment with Houttuynia cordata while TNF-α, MHC-I, C3 and IL-6 were not detected in this herb. There was a minimal difference between Houttuynia cordata, Polygonum cuspidatum and Astragalus spp. treatments in TNF-α gene expression. The result indicated that TNF-α was detected in both treatments of Polygonum cuspidatum and Astragalus spp in treatment groups only. However, in Houttuynia cordata treatment, TNF-α was detected at 10µg/ml only and it was not detected in control groups. Specially, MHC was detected in Astragalus spp. at 1 ug/mL only. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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