Genomic Analyses of the Complete Mitochondrial DNA Sequence of the genus Acrossocheilus (Cyprinidae)
| Autor: | ZHOU,ZHUO-CHENG, 周卓誠 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 In this study, the Acrossocheilus genus, Cyprinidae, is chosen as the main research species due to the uniqueness of geographical distribution compared to other freshwater fish genus. Acrossocheilus is an ideal model to study the correlation between phylogeographical relationship and species distribution. In this study, five species belonging to Acrossocheilus are collected from Zhujiang River and Fujian of mainland China and Taiwan, which are Acrossocheilus paradoxus, Acrossocheilus spinifer, Acrossocheilus beijiangensis, Acrossocheilus jishouensis and Acrossocheilus wuyiensis, and an other specie, Microphysogobio alticorpus are used as outgroup specie in this study. Ten forward and reverse primers are used in identifying each mitochondrial loci. The total complete mitochondrial DNA sequence of species were successfully obtained and submitted to NCBI GenBank. According to the comparative analysis of mitochondria genome, the total length of five Acrossocheilus species and one outgroup specie are estimated to be 16,000 base pairs, including twelve protein-coding genes, two ribosomal RNA genes, and twenty-two transfer genes. By decoding mitochondrial genome, the derived information would be not only helpful on the establishment of molecular systematics database of ichthyology, but understand the speciation mode of Acrossocheilus genus as well as the relationship between Phylogeography and geographic distribution. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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