Establishment a real-time qPCR assay to study the expression of three reproductive-related genes in the oyster drill Thais clavigera
| Autor: | Han-Ting Huang, 黃瀚霆 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Oyster drill (Thais clavigera) is a common intertidal snail in Asia Pacific. It is a widely used bioindicator for monitoring organotin pollution shown as female snail with imposex. However, female skewed sex ratio and reduced penis size in males are observed in Taiwan recently. This phenomenon can’t be explained based on previous morphological and physiological studies. In order to gain better understanding on the abnormality of the oyster drill, the expression of genes in relation to reproduction of aquatic snails including RXR (retinoid X receptor), ER (estrogen receptor) and VTG (vitellogenin) were explored. Therefore, the purpose of this study was to establish a RT-qPCR (real-time quantitative polymerase chain reaction) method to detect the expression level of these three reproductive and development related genes in T. clavigera. By adjusting the reaction conditions and quality checking through gel electrophoresis, the target genes could be amplified specifically. A quantitative method to determine gene expressions of environmental monitoring species T. clavigera was established. Subsequently, variations in different tissues or samples from different sites were evaluated. Moreover, the assessment of the influence of endocrine disruption chemicals on marine snails can be further extended. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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