Measuring masses of single bacterial whole cells with laser induced acoustic desorption plasma charge detection quadrupole ion trap mass spectrometer

Autor:	Shao-Yu Liang, 梁少瑜
Rok vydání:	2016
Druh dokumentu:	學位論文 ; thesis
Popis:	104 The mass measurement of bio-particles with sizes from 50 nm to 3 um is still challenging in mass spectrometric field. In our previous study, we found charge detection quadrupole ion trap mass spectrometer (CD-QIT MS) could measure the mass and mass distribution of cells larger than 3 um with laser induced acoustic desorption (LIAD) method. Here we develop a new LIAD plasma (LIADP) with Ar mix methane gas as ion source to allow ionization of intact bacteria containing several thousands of charge number. We also compare different result by changing experiment condition of Quadrupole Ion Trap (QIT). The individual mass, charge, and mass distribution of polystyrene particles from 2 um to 0.3 um are measured for mass calibration. Escherichia coli and Staphylococcus aureus cells are measured and the average charge is about 1300.
Databáze:	Networked Digital Library of Theses & Dissertations
Externí odkaz:	http://ndltd.ncl.edu.tw/handle/54633426705005600119 Zobrazit plný text záznamu