In vitro analysis of molecular mechanisms underlying effects of Chinese medicine BP011w to inhibit migration of lung adenocarcinoma
| Autor: | Bing-Han Wu, 吳秉翰 |
|---|---|
| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 Lung cancer is one of the most frequently diagnosed cancers and is the major cause of cancer-related death worldwide, as well as in Taiwan. Most patients were diagnosed with advanced-stage lung adenocarcinomas because of its highly metastatic rate. Even if diagnosed at an early stage and surgically removed, lung adenocarcinomas can recurrence and extending to other organs with a high mortality rate after a few months. Recently, Gefitinib and Erlotinib were the most common target therapy for lung cancer, but both of them can’t inhibit the cell migration. Therefore, it is necessary to find a brand new drug against to the metastatic of lung cancer, especially for lung adenocarcinoma. In our study, we want to search a Traditional Chinese Medicines to inhibit cell migration of lung adenocarcinoma because TCMs have lots of advantages including less side effects, naturally, low toxicity, inexpensive than chemotherapy and radiotherapy. After identify by cytological analysis, we found the dosage of 150 μg/ml BP011w could inhibit CL1-5 migration obviously. After analysis the data of microarray, we found 4 candidate genes, DYNAP, FGF16, MIR-548L and REN. In the microarray data, DYNAP, FGF16 and REN were down-regulated; miR-548L was up-regulated after treating by BP011w. DYNAP and MIR-548L were related to PI3K/AKT pathway, FGF16 and REN were related to RAS/ERK pathway. Finally, we surmised that the change of 4 gene expression could block the downstream signal of PI3K/AKT and RAS/ERK due to the treatment of BP011w. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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