Physiological function of GDSL-lipase in rice (Oryza sativa)

Autor: Chih-Ming Chiu, 邱志明
Rok vydání: 2016
Druh dokumentu: 學位論文 ; thesis
Popis: 104
GDSL-lipases are hydrolytic enzymes, widely present in different organisms, such as microorganism, fungi and plants. Unlike the common lipases, GDSL lipases has a distinct GDSL motif with a serine-containing active site near the N-terminus and have four invariant catalytic residues Ser, Gly, Asn and His in conserve blocks I, II, III, and V, respectively. Biochemical functions of various GDSL-lipases had been characterized, that can hydrolysis and synthesis important ester compounds. GDSL-lipases were used to change the properties of lipids and applied to pharmaceutical, food, and biochemical catalytic process. But the physiological functions of GDSL-lipases are still unclear. GDSL-lipases were a gene family, at least 114 GDSL-lipases in the rice genome, while the role of GDSL-lipases are still unclear in rice. In this study, the rice materials were purchased from the Taiwan Rice Insertion Mutants (TRIM) database created by Academician Dr. Yu’s team. We used the keyword, GDSL-lipase, to screan the mutant lines from TRIM database. We selected the lines with T-DNA insertion on or near by rice GDSL-lipase gene and cultured them to germinate the T3 seed for zygous detection. From 90 mutant lines, we got 32 homozygous lines for futher salt stress treatment. Observation the trait after treatment, we obtained three salt tolerance mutant lines: YTC-99, YTC-110, YTC-111. In further study, the T4 progeny plants were used for enzyme active assay. In this study, we used salt tolerance and GDSL-lipase over expression mutant lines to investigate the association between salt tolerance and GDSL-lipases expression levels. As the result, the H2O2 contents were reduced in all the salt tolerance mutant lines. However the enzyme active assays indicated that the peroxidase and Mn-SOD activities were indicued in mutant lines; while the activities of catalase, ascorbate peroxidase, superoxide dismutase and glutathione redutase were not effected. Therefore, the pathway of GDSL-lipase involving the salt tolerance needs further study.
Databáze: Networked Digital Library of Theses & Dissertations
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