Protective effects of Astaxanthin on Homocysteine and Glutamate-induced neurotoxicity in PC12 cells
| Autor: | Guo-Jun Liao, 廖國均 |
|---|---|
| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 This research paper using Homocysteine and Glutamate induced cytotoxicity of PC12 cell line, and investigate the Astaxanthin effect of their protection.In humans, Homocysteine is a metabolic intermediate of methionine. Adverse metabolic effects have high Homocysteine concentrations. Homocysteine is easily oxidized to form reactive oxygen species, generated cytotoxicity.Or through a series of reaction to Homocysteine thiolactone, leading to protein misfolding and cause endoplasmic reticulum stress, when ER stress is not eliminated, it will lead to apoptosis. Glutamate is the major excitatory neurotransmitter in the brain, the only excess Glutamate release will cause overstimulation of the NMDA receptor, the Ca2+move within the cell, trigger cell excitotoxicity. In this paper, using the method of assessment for the survival of PC12 cells, Production of ROS, the intracellular calcium content, MDA lipid peroxide content, the apoptosis-related proteins such as Bax, Bcl-2, caspase-12 and caspase-3 expression changes. Experimental results show that Homocysteine, Glutamate can effectively induce injury and cause damage bonus effect in PC12 cells. when added Astaxanthin, PC12 cells viability were improved, ROS and intracellular calcium also inhibited, effectively reduce the content of MDA.Analysis of the apoptosis-related proteins on Bax, Bcl-2, caspase-12 and caspase-3 expression changes. It was found that Astaxanthin can reduce apoptotic protein Bax and Caspase-3, enhance the anti-apoptotic protein Bcl-2, etc., protect cells from apoptosis and increase the survival rate of the cells.Caspase-12 performance variation proved that Homocysteine and Glutamate can cause endoplasmic reticulum stress. Key words: Homocysteine, Glutamate, Astaxanthin, PC12 cells. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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