Manufacturing and Properties evaluation of the polyethylene oxide/water-solubility Chitosan electrospinning nanofiber membranes
| Autor: | Szu-Chieh Huang, 黃思傑 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 In this study, polyethylene oxide (PEO) and water-soluble chitosan (WCS) are combined using an electrospinning technique. Both materials have satisfactory biocompatibility, hygroscopicity, and antibacterial properties, and are suitable candidates for use in medical treatment and food processing packaging. PEO and WCS are blended and formed formed into nanofiber membranes, during which the fiber formation is manipulated using different spinning voltages and electrospinning solution consistencies. The optimal electrospinning parameters are thus determined in terms of the viscosity and electrical conductivity. Afterwards, the optimal parameters are combined with the PEO/WCS blends along with the addition of Tetracycline hydrochloride (TTC-HCL) as a medical ingredient, yielding PEO/WCS/TTC-HCL nanofiber membranes. Meanwhile, PEO/WCS/TTC-HCL composite films are made using casting molding. These nanofiber membranes and composite films are compared in terms of the antibacterial properties against Staphylococcus aureus (S. aureus) and Escherichia coli (E. coli) via a quantitative antimicrobial test and MTT assay test. The test results indicate that pure PEO solution has a greater viscosity than pure WCS solution; however, the latter has a greater electrical conductivity. The viscosity of PEO/WCS blends decreases as a result of more WCS content. When the blending ratio is 40/60, PEO/WCS blend has a viscosity of 2190 cp and an electrical conductivity of 2304μs/cm. Scanning electron microscopy (SEM) observation confirms that this specified blending ratio can yield the optimal nanofiber formation. In addition, Fourier Transform infrared spectroscopy (FT-IR) results show that PEO and WCS blends have a physical mixing. The optimal parameters are PEO/WCS ratio of 40/60 and a consistency of 8wt%. The resulting PEO/WCS/TTC-HCL nanofiber membranes exhibit greater water contact angle than PEO/WCS/TTC-HCL composite films, indicating a higher drug release capability. Similarly, PEO/WCS/TTC-HCL nanofiber membranes also exhibit greater anti-bacterial properties against S. aureus and E. coli than PEO/WCS/TTC-HCL composite films. Neither nanofiber membranes nor composite films decrease cell activity. This study successfully prepares PEO/WCS nanofiber membranes and the addition of TTC-HCL provides the membranes with antibacterial efficacy. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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