Insulin-contained biodegradable polymer microneedle patches in drug delivery
| Autor: | Yi Jia Tzeng, 曾翊嘉 |
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| Rok vydání: | 2016 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 104 In this study, the two-layer biodegradable polymer microneedle patches (MNs) were fabricated by using centrifugal method. Polycaprolactone (PCL) and polyethylene glycol (PEG) acted to the major materials in making the MNs and sodium carboxymethyl cellulose (CMC) was the supporting array. The MNs were used to evaluate the transdermal delivery of insulin. The stereomicroscope and scanning electron microscope were used to compare the shapes of 3MTM master structure and the two-layered insulin-loaded MNs. The results showed that the two-layered insulin-load MNs lost about 1.2 % of the height but both MNs still had the similar shape. The encasement of FITC-labeled insulin was verified by using the fluorescence microscopy. The results of in vitro puncture study showed that when MNs were inserted into the porcine cadaver skin, a high insertion ratio (ca. 100%) independent of the drug loading was obtained. The Hematoxylin and Eosin staining (H&E staining) investigation showed that MNs could pierce into the porcine cadaver skin at approximately 232 μm depth. An in vitro transdermal study by using confocal laser scanning microscope (CLSM) showed that the encapsulated FITC-insulin gradually diffused in the dermal layer, reaching a penetration depth of 320 μm. The results of in vitro transdermal penetration study in Franz diffusion cells revealed that the molecular weight and loading of the drug would affect the release profile, which may be employed to predict the release model of another drug. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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