The Roles of Apoptotic Gene caspase-3/7 on Anterior Regeneration in Aeolosoma viride
| Autor: | Yi-Hua Chiang, 蔣宜樺 |
|---|---|
| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 In recent years, regeneration is a hot issue. When animal tissues or organs are damaged or lost, the processes of cell proliferation and differentiation can repair the damaged tissues or organs helping the animal to survive. Several animal species have been used to investigate regeneration, including hydra, planarian, salamander and zebrafish. Previous studies showed that when animals are wounded, the programmed cell death, apoptosis, would happen at the wound site before regeneration occurred. It is well known that apoptosis is a homeostatic mechanism for removing the damaged cells. In apoptosis, caspase-3 and caspase-7 are required functional genes to kill cells. Although the relationship between apoptosis and regeneration had been documented, their linkage was not clearly demonstrated. Aeolosoma viride is a fresh water annelid, which has been used as a model organism for studying regeneration recently. When its anterior region was amputated, the whole anterior part could completely regenerate within five days. In this study, a caspase gene of A.viride was cloned, and its nucleotide sequence is similar to caspase-3 and caspase-7. Therefore, it was named Avi-cas-3/7. The results indicated that Avi-cas-3/7 participated with anterior regeneration in A. viride. It showed a specific expressed pattern during regenerative process. Its gene expressions had increased and decreased twice before the end of regeneration. This result was inferred that the first peak of Avi-cas-3/7 expression might involve in removing the damaged cells, and the second peak might relate to pattern formation of anterior region in which the extra cells were removed during regeneration. Furthermore, Avi-cas-3/7 expression was knocked down by RNA interference (RNAi), and anterior regeneration was inhibited. This result indicated that Avi-cas-3/7 has a crucial role in regeneration in A. viride. In summary, apoptotic gene, Avi-cas-3/7 is actually involved in the anterior regeneration in A. viride. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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