Lipid composition for freeze-tolerance at different seasons and altitudes in the earthworms

Autor: Yu-Chih Lo, 羅友志
Rok vydání: 2015
Druh dokumentu: 學位論文 ; thesis
Popis: 103
The aim of my research is to understand the relationship between the composition changes of cellular lipids in earthworms and their cold resistance. It has been well known that the lipid bilayer membrane with more unsaturated fatty acid including EPA (Eicosapentaenoic acid) & DHA (Docosahexaenoic acid) can resist lower temperature damage. Therefore the fatty acid composition of cell membrane is a suitable index to evaluate the ability of cold resistancen in animals. To find out whether does the earthworm’s cellular lipids compositions change in low temperature, earthworms were collocated from different seasons and different altitudes, or were kept and acclimated at different temperatures. The results showed that Amynthas tessellatus tessellatus has higher proportion of unsaturated fatty acids and lower the proportion of saturated fatty acids at low temperature. Especialy, the amount of DHA in A. tessellatus tessellatus from high altitude was higher than that from media altitude. Also, the amount of DHA in those earthworms collected from winter time was higher than that from summer time. The total fatty acid composition in Pontoscolex corethrurus had no significant difference under different temperature acclimatuon. Because Pontoscolex corethrurus does not have physiological mechanism to regulate their fatty acid composition during temperature changing situation. It might infer that Pontoscolex corethrurus should be difficult to spread to higher altitude areas in Taiwan.
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