Study of Min Protein-Induced Membrane Species Waves in vitro
| Autor: | Yu-Ming Tu, 杜育銘 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 In the bacterium Escherichia coli, the proper placement of the division site selection is regulated in part by the pole-to-pole oscillations of Min proteins. The oscillation dynamics emerges from the self-organization of MinD, MinE and ATP in vivo, which also forms planar surface waves on supported lipid bilayer in vitro. However, it is still unclear how the Min proteins affect the E. coli lipid membrane and interact with the other E. coli membrane proteins, even though how Min proteins interact with the membrane surface to generate the in vivo oscillations is a subject of intense study. We hypothesized that the spatial oscillations of Min protein systems could play a crucial role in changing lipid membrane dynamics and therefore indirectly influence the dynamics of other membrane associated species. We reconstituted the system on supported E. coli lipid bilayer platforms in order to systematically explore the underlying mechanism between lipid membranes and Min proteins. We observed fluorescently labeled lipids in the E. coli membranes moving in spiral waves after the addition of unlabeled MinD, MinE and ATP to the membranes. Fluorescence recovery after photobleaching (FRAP) and kymograph analyses showed that dynamics of the labeled lipid membrane waves had different characteristics from those of the Min protein waves. In the future, some E. coli signaling membrane proteins could be incorporated into the membrane to examine whether their movement or clustering can be influenced by the Min proteins-induced membrane waves. The result could provide us insights into the long-standing question about other possible function of the E. coli Min protein oscillation phenomenon. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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