A Robust Design of Quorum Sensing Symbiosis Ecosystem Controlled by Small Molecule Regulator through Cell-Cell Communication
| Autor: | Wang, Chih Chieh, 王致捷 |
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| Rok vydání: | 2015 |
| Druh dokumentu: | 學位論文 ; thesis |
| Popis: | 103 Microbial ecosystem plays a key role in maintaining Earth’s biosphere and supporting lives. Many researches have shown interaction between environment and microbial ecosystem, but the complexity and structure of natural microbial ecosystem still need to be clearly described. In this study, we construct a controllable symbiosis ecosystem to provide different levels of mutualistic behaviors through cell-cell communication. With the well-characterized kinetic components and the detailed mathematical analysis of the synthetic symbiosis ecosystem, we can provide insights into the natural functions and the complex interactions among the genetic networks, environmental effect, and system behavior of symbiosis ecosystem. Based on different promoter-RBS libraries and steady state model of symbiosis of ecosystem, we develop a design procedure to select a set of adequate promoter-RBS components from the corresponding libraries to achieve a robust synthetic symbiosis ecosystem with a desired mutualistic behavior despite intrinsic parametric variations and environmental disturbances. Therefore, we believe that the proposed controllable symbiosis ecosystem may acts as a chassis for constructing more complicated microbial ecosystem in the fast growing field of synthetic biology. |
| Databáze: | Networked Digital Library of Theses & Dissertations |
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